Hyperbaric oxygen in skeletal muscle of rats submitted to total acute left hindlimb ischemia: A research report

Determine the effect of hyperbaric oxygen treatment in skeletal muscle of rats submitted to total acute left hindlimb ischemia. An experimental study was designed using 48 Wistar rats divided into four groups (n = 12): Control; Ischemia (I)--total hindlimb ischemia for 270 minutes; Hyperbaric oxygen...

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Published inUndersea & hyperbaric medicine Vol. 42; no. 1; p. 41
Main Authors da Silva, Luis Gustavo Campos, Dalio, Marcelo Bellini, Joviliano, Edwaldo Edner, Feres, Omar, Piccinato, Carlos Eli
Format Journal Article
LanguageEnglish
Published United States 01.01.2015
Subjects
Acute Disease
Animals
Aspartate Aminotransferases - analysis
Creatine Kinase - analysis
Glycogen - analysis
Hindlimb - blood supply
Hyperbaric Oxygenation
Ischemia - metabolism
Ischemia - therapy
L-Lactate Dehydrogenase - analysis
Male
Malondialdehyde - analysis
Muscle, Skeletal - blood supply
Muscle, Skeletal - metabolism
Random Allocation
Rats
Rats, Wistar
Research Report
Serum Albumin - analysis
Time Factors
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Summary:Determine the effect of hyperbaric oxygen treatment in skeletal muscle of rats submitted to total acute left hindlimb ischemia. An experimental study was designed using 48 Wistar rats divided into four groups (n = 12): Control; Ischemia (I)--total hindlimb ischemia for 270 minutes; Hyperbaric oxygen treatment during ischemia (HBO2)--total hindlimb ischemia for 270 minutes and hyperbaric oxygen during the first 90 minutes; Pre-treatment with hyperbaric oxygen (PHBO2)--90 minutes of hyperbaric oxygen treatment before total hindlimb ischemia for 270 minutes. Skeletal muscle injury was evaluated by measuring levels of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total creatine phosphokinase (CPK); muscular malondialdehyde (MDA), muscular glycogen, and serum ischemia-modified albumin (IMA). AST was significantly higher in I, HBO2 and PHBO2 compared with control (P = .001). There was no difference in LDH. CPK was significantly higher in I, HBO2 and PHBO2, compared with control (p = .014). MDA was significantly higher in PHBO2, compared with other groups (p = .042). Glycogen was significantly decreased in I, HBO2 and PHBO2, compared with control (p < .001). Hyperbaric oxygen treatment in acute total hindlimb ischemia exerted no protective effect on muscle injury, regardless of time of application. When applied prior to installation of total ischemia, hyperbaric oxygen treatment aggravated muscle injury.
ISSN:1066-2936