Photobiomodulation Therapy for Attenuating the Dystrophic Phenotype of Mdx Mice

• Published in: Photochemistry and photobiology Vol. 96; no. 1; pp. 200 - 207
• Main Authors: Macedo, Aline Barbosa, Mizobuti, Daniela Sayuri, Hermes, Tulio de Almeida, Mâncio, Rafael Dias, Pertille, Adriana, Kido, Larissa Akemi, Cagnon, Valéria Helena Alves, Minatel, Elaine
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.01.2020
• Subjects: Aluminum; Angiogenesis; Animals; Arsenides; Disease control; Duchenne's muscular dystrophy; Dystrophy; Gallium; Gallium arsenide; Genotype & phenotype; Inflammation; Light therapy; Low-Level Light Therapy; Male; Markers; Mice; Mice, Inbred C57BL; Mice, Inbred mdx; Muscles; Muscular dystrophy; Muscular Dystrophy, Animal - metabolism; Muscular Dystrophy, Animal - pathology; Muscular Dystrophy, Animal - therapy; Muscular Dystrophy, Duchenne - metabolism; Muscular Dystrophy, Duchenne - pathology; Muscular Dystrophy, Duchenne - therapy; Musculoskeletal system; MyoD protein; Oxidative stress; Parameters; Phenotype; Phenotypes; Prednisolone; Quadriceps muscle; Regeneration; Rodents; Skeletal muscle; Therapy; Transforming Growth Factor beta - metabolism; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism
• ISSN: 0031-8655; 1751-1097
• DOI: 10.1111/php.13179

This study analyzed photobiomodulation therapy (PBMT) effects on regenerative, antioxidative, anti‐inflammatory and angiogenic markers in the dystrophic skeletal muscle of mdx mice, the experimental model of Duchenne muscular dystrophy (DMD), during the acute phase of dystrophy disease. The following groups were set up: Ctrl (control group of normal wild‐type mice; C57BL/10); mdx (untreated mdx mice); mdxPred (mdx mice treated with prednisolone) and mdxLA (mdx mice treated with PBMT). The PBMT was carried out using an Aluminum Gallium Arsenide (AIGaAs; IBRAMED® laserpulse) diode, 830 nm wavelength, applied on the dystrophic quadriceps muscle. The mdxLA group showed a degenerative and regenerative area reduction simultaneously with a MyoD level increase, ROS production and inflammatory marker reduction and up‐regulation in the VEGF factor. In addition, PBMT presented similar effects to prednisolone treatment in most of the parameters analyzed. In conclusion, our results indicate that PBMT in the parameters selected attenuated the dystrophic phenotype of mdx mice, improving skeletal muscle regeneration; reducing the oxidative stress and inflammatory process; and up‐regulating the angiogenic marker. The photobiomodulation (PBM; Aluminum Gallium Arsenide diode, 830 nm wavelengths) was applied on the dystrophic quadriceps muscle of mdx mice, the experimental model of Duchenne muscular dystrophy, during the acute phase of dystrophy disease. PBM attenuated the dystrophic phenotype of mdx mice, reducing the degenerative, inflammatory and oxidative stress markers. At the same time, the PBM promoted the increase of angiogenesis mediator.