On‐line preconcentration methods for capillary electrophoresis

• Published in: Electrophoresis Vol. 21; no. 14; pp. 2768 - 2779
• Main Authors: Osbourn, Damon M., Weiss, David J., Lunte, Craig E.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.08.2000
• Subjects: Animals; Capillary electrophoresis; Electrophoresis, Capillary - methods; Field‐amplified stacking; Humans; On‐line preconcentration; pH‐mediated stacking; Review; Stacking
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/1522-2683(20000801)21:14<2768::AID-ELPS2768>3.0.CO;2-P

The limits of detection (LOD) for capillary electrophoresis (CE) are constrained by the dimensions of the capillary. For example, the small volume of the capillary limits the total volume of sample that can be injected into the capillary. In addition, the reduced pathlength hinders common optical detection methods such as UV detection. Many different techniques have been developed to improve the LOD for CE. In general these techniques are designed to compress analyte bands within the capillary, thereby increasing the volume of sample that can be injected without loss of CE efficiency. This on‐line sample preconcentration, generally referred to as stacking, is based on either the manipulation of differences in the electrophoretic mobility of analytes at the boundary of two buffers with differing resistivities or the partitioning of analytes into a stationary or pseudostationary phase. This article will discuss a number of different techniques, including field‐amplified sample stacking, large‐volume sample stacking, pH‐mediated sample stacking, on‐column isotachophoresis, chromatographic preconcentration, sample stacking for micellar electrokinetic chromatography, and sweeping.