Influence of estradiol analogue on cell growth, morphology and death in esophageal carcinoma cells

2-Methoxyestradiol-bis-sulphamate is a bis-sulphamoylated derivative of the naturally occurring 17-beta-estradiol metabolite namely 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate is regarded as a potential anticancer drug with increased antiproliferative activity when compared to 2-methoxyest...

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Bibliographic Details
Published inBiocell Vol. 34; no. 3; pp. 113 - 120
Main Authors Mqoco, Thandi, Marais, Sumari, Joubert, Annie
Format Journal Article
LanguageEnglish
Portuguese
Published Argentina Tech Science Press 01.12.2010
Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
Subjects
17β-Estradiol
Apoptosis
Apoptosis - drug effects
Autophagy
Autophagy - drug effects
BIOLOGY
Carcinoma, Squamous Cell - pathology
Cell Count
Cell Cycle - drug effects
Cell death
Cell Division - drug effects
Cell growth
Cell Line, Tumor
Cell morphology
Cytology
Dose-Response Relationship, Drug
Esophageal cancer
Esophageal carcinoma
Esophageal Neoplasms - pathology
Esophagus
Estriol - analogs & derivatives
Estriol - pharmacology
Humans
Light microscopy
Metabolites
Microscopy
Microscopy, Interference
Morphology
Phagocytosis
Pilot Projects
Staining and Labeling
Autophagy
Cancer
Apoptosis
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Summary:2-Methoxyestradiol-bis-sulphamate is a bis-sulphamoylated derivative of the naturally occurring 17-beta-estradiol metabolite namely 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate is regarded as a potential anticancer drug with increased antiproliferative activity when compared to 2-methoxyestradiol. The aim of this pilot in vitro study was to determine the influence of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and possible induction of certain types of cell death in the SNO esophageal carcinoma cell line. A dose-dependent study (0.2-1.0 microM) was conducted with an exposure time of 24 hours. Data revealed that 2-methoxyestradiol-bis-sulphamate reduced cell numbers statistically significantly to 74% after exposure to 0.4 microM of the drug. Morphological studies including light microscopy demonstrated hallmarks of apoptosis, while fluorescent microscopy revealed both the presence of apoptosis and autophagy as types of cell death being induced in SNO cells after 24 hours of exposure to 0.4 microM 2-methoxyestradiol-bis-sulphamate.
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ISSN:0327-9545
1667-5746