The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics

Multiprotein complexes regulate most if not all cellular functions. Elucidating the structure and function of these complex cellular machines is essential for understanding biology. Moreover, multiprotein complexes by themselves constitute powerful reagents as biologics for the prevention and treatm...

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Bibliographic Details
Published inAdvances in experimental medicine and biology Vol. 896; p. 199
Main Authors Sari, Duygu, Gupta, Kapil, Thimiri Govinda Raj, Deepak Balaji, Aubert, Alice, Drncová, Petra, Garzoni, Frederic, Fitzgerald, Daniel, Berger, Imre
Format Journal Article
LanguageEnglish
Published United States 01.01.2016
Subjects
Animals
Baculoviridae - genetics
Baculoviridae - metabolism
Computational Biology
Databases, Protein
Drug Discovery - methods
Gene Expression Regulation, Viral
Genetic Vectors
Humans
Models, Molecular
Multiprotein Complexes
Protein Engineering - methods
Protein Multimerization
Protein Structure, Quaternary
Protein Subunits
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Structure-Activity Relationship
Transcription, Genetic
Transfection
Viral Proteins - biosynthesis
Viral Proteins - chemistry
Viral Proteins - genetics
X-ray crystallography
Gene transfer
Lepidoptera
MultiBac
Synthetic biology
Vaccines
Baculovirus
Electron microscopy
Insect cell expression system
Structural biology
Virus-like particles (VLPs)
Protein complexes
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Summary:Multiprotein complexes regulate most if not all cellular functions. Elucidating the structure and function of these complex cellular machines is essential for understanding biology. Moreover, multiprotein complexes by themselves constitute powerful reagents as biologics for the prevention and treatment of human diseases. Recombinant production by the baculovirus/insect cell expression system is particularly useful for expressing proteins of eukaryotic origin and their complexes. MultiBac, an advanced baculovirus/insect cell system, has been widely adopted in the last decade to produce multiprotein complexes with many subunits that were hitherto inaccessible, for academic and industrial research and development. The MultiBac system, its development and numerous applications are presented. Future opportunities for utilizing MultiBac to catalyze discovery are outlined.
ISSN:0065-2598
DOI:10.1007/978-3-319-27216-0_13