Studies of the antigenicity and allergenicity of phospholipase A2 of bee venom

The antigenic and allergenic properties of phospholipase A2 (PLA2) and whole bee venom were compared by measuring the IgG and IgE antibody responses in animals and man. Precipitating antibodies raised in rabbits and reaginic and other antibodies raised in mice reacted about equally with both bee ven...

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Bibliographic Details
Published inJournal of allergy and clinical immunology Vol. 58; no. 2; p. 322
Main Authors Light, W C, Reisman, R E, Ilea, V S, Wypych, J I, Okazaki, T, Arbesman, C E
Format Journal Article
LanguageEnglish
Published United States 01.01.1976
Subjects
Allergens - analysis
Animals
Antibody Specificity
Bees
Histamine Release
Humans
Immunoglobulin E - analysis
Passive Cutaneous Anaphylaxis
Phospholipases - immunology
Precipitins - analysis
Rabbits
Radioimmunoassay
SRS-A - analysis
Venoms - immunology
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Summary:The antigenic and allergenic properties of phospholipase A2 (PLA2) and whole bee venom were compared by measuring the IgG and IgE antibody responses in animals and man. Precipitating antibodies raised in rabbits and reaginic and other antibodies raised in mice reacted about equally with both bee venom and PLA. The majority of human sera containing bee venom-specific IgE also contained PLA-specific IgE, although in somewhat lower titers. Similarly, most human sera with significant amounts of total antibodies reacting with bee venom also had antibodies reacting with PLA. Histamine and SRS-a release from leukocytes of sensitive patients followed challenge with whole bee venom and PLA in the majority of instances. However, mediator release from several patients' cells was obtained with bee venom only. These studies suggest that although PLA is a major allergen and antigen in bee venom, significant exceptions in patients' reactivity may limit its potential diagnostic and therapeutic usefulness.
ISSN:0091-6749
DOI:10.1016/0091-6749(76)90138-X