Detection of Vimentin (VIM) Methylation in the Serum of Colorectal Cancer Patients

Detection of colorectal cancer using serum assay of vimentin methylation. We attempted to detect vimentin methylation in the serum of colorectal cancer patients using quantitative methylation-specific polymerase chain reaction (qMSP). Of 44 colorectal cancer patients, 4 (9%) exhibited methylation of...

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Bibliographic Details
Published inAnticancer research Vol. 30; no. 12; pp. 5015 - 5018
Main Authors SHIRAHATA, Atsushi, SAKURABA, Kazuma, GOTO, Tetsuhiro, SAITO, Mitsuo, ISHIBASHI, Kazuyoshi, KIGAWA, Gaku, NEMOTO, Hiroshi, HIBI, Kenji
Format Journal Article
LanguageEnglish
Published Attiki International Institute of Anticancer Research 01.12.2010
Subjects
Aged
Biological and medical sciences
Colorectal Neoplasms - blood
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
DNA Methylation
DNA, Neoplasm - blood
DNA, Neoplasm - genetics
Female
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Male
Medical sciences
Middle Aged
Polymerase Chain Reaction
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
Vimentin - blood
Vimentin - genetics
Human
Vimentin
Rectal disease
Colorectal carcinoma
Colorectal cancer
Malignant tumor
quantitative methylation-specific PCR
Colonic disease
Polymerase chain reaction
Cancerology
Digestive diseases
Intestinal disease
Serum
Diagnosis
VIM
Molecular biology
Methylation
Cancer
Quantitative analysis
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Summary:Detection of colorectal cancer using serum assay of vimentin methylation. We attempted to detect vimentin methylation in the serum of colorectal cancer patients using quantitative methylation-specific polymerase chain reaction (qMSP). Of 44 colorectal cancer patients, 4 (9%) exhibited methylation of the vimentin gene in their serum DNA by qMSP. Interestingly, methylation was significantly found in the serum of patients with liver metastasis, peritoneal dissemination, and distant metastasis (p = 0.026, p = 0.0029 and p = 0.0063, respectively), suggesting that vimentin methylation in serum might be detected more frequently in patients with advanced colorectal cancer. The high sensitivity of qMSP makes it possible to detect smaller amounts of tumor DNA in the serum, suggesting that qMSP can be used as a screening method for cancer.
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ISSN:0250-7005
1791-7530