Degradation of polychlorinated biphenyls by hairy root culture of Solanum nigrum

• Published in: Biotechnology letters Vol. 19; no. 8; pp. 787 - 790
• Main Authors: MACKOVA, M, MACEK, T, KUCEROVA, P, BURKHARD, J, PAZLAROVA, J, DEMNEROVA, K
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.08.1997; Springer Nature B.V
• Subjects: Biodegradation of pollutants; Biological and medical sciences; Biotechnology; Environment and pollution; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; PCB; Polychlorinated biphenyls; Biodegradation; Cell proliferation; Hairy root; Vegetals; Tissue culture; Environmental factor; Polychlorobiphenyl; Phytoremediation; Pollutant; Solanum nigrum; Dicotyledones; Chlorine Organic compounds; Angiospermae; Plant growth substance; Spermatophyta; Aromatic compound; Solanaceae
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1023/A:1018348511978

Polychlorinated biphenyls (PCBs) present in the commercial mixture Delor 103 were transformed by hairy root culture of Solanum nigrum. Plant growth regulators kinetin, 2,4-dichlorophenoxy-acetic acid, benzylaminopurin and/or naph-thaleneacetic acid, influenced the cells' growth and transformation of PCBs in a different manner. The cells were able to transform PCBs even if they ceased growing. Young inoculum (16 days) had a 20% lower PCB conversionthan did older inocula (37, 68 days) while biomass increase was much higher using young inoculum. With increasing size of inoculum, transformation of PCBs was stimulated. After 30 days of incubation the average amount of residual PCBs was 40% of the controls at initial PCB concentration of 100 ppm.[PUBLICATION ABSTRACT]