Molecular characteristics of HIV type 1 circulating in São Paulo, Brazil

• Published in: AIDS research and human retroviruses Vol. 21; no. 7; p. 673
• Main Authors: Brígido, Luis F M, Franco, Heitor M, Custódio, Renata M, Oliveira, Carmen A F, P Ferreira, João Leandro, Eira, Margareth, Bergel, Fernando, Araújo, Fabio, Carvalheiro, Jose R, Rodrigues, Rosangela
• Format: Journal Article
• Language: English
• Published: United States 01.07.2005
• Subjects: Amino Acid Sequence; Base Sequence; Brazil; DNA Primers; Female; Gene Products, env - genetics; Gene Products, gag - genetics; Gene Products, pol - genetics; HIV Infections - virology; HIV-1 - classification; Humans; Male; Molecular Sequence Data; Phylogeny; Sequence Homology, Amino Acid; Brazil
• ISSN: 0889-2229
• DOI: 10.1089/aid.2005.21.673

Partial sequences of HIV-1 polymerase from 185 patients, 141 ARV experienced and 44 naive, of gag (p24) and env (C2V3) from a subset of naive cases were evaluated in São Paulo, Brazil. Antiretroviral resistance mutations were detected in 4% of 26 recently (<2 years) infected patients. Polymorphisms at the protease gene were common both in contemporary and pre-HAART era isolates, some significantly associated with the viral clade. HIV-1 clade B was preponderant, in 79%, with 11% clade F and one case of HIV-1 C. Recently infected women had a significantly higher proportion of non-B clade HIV-1. A mosaic pol was observed in 9%, all B/F except for one G mosaic. A CRF-12-BF structure, observed in 20% of B/F pol mosaics, provides evidence for an epidemic relationship in the major South American metropolitan areas.