Diffusion of CO2 and other gases inside leaves

• Published in: The New phytologist Vol. 126; no. 3; pp. 449 - 479
• Main Author: PARKHURST, DAVID F.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.1994; Blackwell
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Intercellular diffusion; leaf anatomy; mesophyll conductance; Metabolism; photosynthesis models; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; stomata; transport resistances; Assimilation; Resistance; Vegetals; Gaseous diffusion; Intercellular space; Carbon dioxide; Stomata; Plant leaf; Review; Anatomy; Photosynthesis
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/j.1469-8137.1994.tb04244.x

SUMMARY Diffusion of CO2 in the intercellular airspaces of the leaf mesophyll is one of the many processes that can limit photosynthetic carbon assimilation there. This limitation has been largely neglected in recent years, but both theoretical and empirical evidence is presented showing that it can be substantial, reducing CO2 assimilation rates by 25% or more in some leaves. Intercellular diffusion is fundamentally a three‐dimensional process, because CO2 enters the leaf through discrete stomata, and not through a uniformly porous epidermis. Modelling it in one dimension can cause major underestimation of its limiting effects. Resistance and conductance models often fail to account well for the limitation, in part because they are usually one‐dimensional representations, but also because they treat continuously interacting processes as if they were sequential. A three‐dimensional diffusion model is used to estimate the intercellular limitation in leaves of different structural types. Intercellular gaseous diffusion probably limits CO2 assimilation by at most a few percent in many of the agricultural plants commonly studied by laboratory physiologists, as they usually have thin, amphistaomatous leaves. However, it may cause substantial limitations in the thicker, often hypostomatous leaves of the wild plants that occupy large areas of the earth. Several physiological implications of intercellular diffusion are discussed. I close by reiterating published suggestions that the gas‐exchange parameter commonly termed pi should be renamed pes (es for evaporating surfaces) to avoid the confusing implication that p1, is‘the’intercellular CO2 pressure. Contents Summary 449 I. Introduction 450 II. Theory of intercellular gaseous diffusion 451 III. Evidence regarding intercellular gaseous diffusion hmitations 461 IV. Discussion of assumptions made in §11.1 470 V. Physiological implications of intercellular gaseous diffusion 471 VI. Conclusions 475 Acknowledgements 476 Appendix, Example showing breakdown of resistance analogy for diffusion with distributed uptake 476 References 477