Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients

• Published in: Current medicinal chemistry Vol. 9; no. 8; pp. 793 - 797
• Main Authors: BOOR, K, RONAI, Z, NEMODA, Z, GASZNER, P, SASVARI-SZEKELY, M, GUTTMAN, A, KALASZ, H
• Format: Journal Article
• Language: English
• Published: Oak Park, IL Bentham Science 01.04.2002
• Subjects: Addictive behaviors; Adult and adolescent clinical studies; Base Sequence; Biological and medical sciences; DNA Primers; DRD4 gene; Drug addiction; Genotype; Humans; Medical sciences; Nanotechnology; Psychology. Psychoanalysis. Psychiatry; Psychopathology. Psychiatry; Receptors, Dopamine D2 - genetics; Receptors, Dopamine D4; Substance-Related Disorders - genetics; Human; D4 Dopamine receptor; Drug addiction; Typing; DNA; Non invasive method; Genotype; Drug of abuse; Sampling; Genetic determinism; Heroin
• ISSN: 0929-8673; 1875-533X
• DOI: 10.2174/0929867024606821

A noninvasive DNA sampling method has been implemented collecting buccal mucosa cells by cotton wool swabs. An amount of 0.2 2 microg DNA per patient was obtained after the phenol-extraction procedure and 0.2 2 ng DNA template was sufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopamine receptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separation by laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drug-dependent subjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variants beside the common 4-repeat DRD4 allele.