Comparative analysis of alternating purine-pyrimidine sites in yeast chromosomes

It is known that under some circumstances DNA sequences made up of purine and pyrimidines in repeated alternation can undergo a transition from the right-handed to a left handed helical configuration which has been called Z-DNA. We have analyzed yeast chromosomal DNAs for the presence of alternating...

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Bibliographic Details
Published inYeast (Chichester, England) Vol. 5 Spec No; p. S297
Main Authors Rollo, F, Vaughan Martini, A, Martini, A, Pacilli, A, Ferracuti, T, Amici, A
Format Journal Article
LanguageEnglish
Published England 01.04.1989
Subjects
Autoradiography
Blotting, Southern
Densitometry
DNA Probes
DNA, Fungal - genetics
Electrophoresis, Agar Gel
Nucleic Acid Hybridization
Purine Nucleotides
Pyrimidine Nucleotides
Saccharomyces cerevisiae - genetics
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Summary:It is known that under some circumstances DNA sequences made up of purine and pyrimidines in repeated alternation can undergo a transition from the right-handed to a left handed helical configuration which has been called Z-DNA. We have analyzed yeast chromosomal DNAs for the presence of alternating purine-pyrimidine stretches using a new strategy. Our strategy takes advantage of pulsed field gel electrophoresis (PFGE) to separate yeast chromosomal DNAs. They are subsequently analyzed by molecular hybridization with radioactive (dC-dA)n.(dG-dT)n or (dG-dC)n.(dC-dG)n probes, autoradiography and densitometric scanning. Preliminary results obtained by the application of the new method to Saccharomyces cerevisiae are described.
ISSN:0749-503X