The use of comparative duplex PCR in monitoring of patients with non-Hodgkin's lymphoma and chronic lymphocytic leukaemia

• Published in: Folia biologica Vol. 51; no. 6; pp. 177 - 179
• Main Authors: Slavícková, A, Forsterová, K, Ivánek, R, Cerný, J, Klener, P
• Format: Journal Article
• Language: English
• Published: Czech Republic Charles University in Prague, First Faculty of Medicine 01.01.2005
• Subjects: Genetic Markers; Humans; Leukemia, Lymphocytic, Chronic, B-Cell - metabolism; Leukemia, Lymphocytic, Chronic, B-Cell - therapy; Lymphoma, Non-Hodgkin - therapy; Polymerase Chain Reaction - methods; Prognosis; Treatment Outcome
• ISSN: 0015-5500; 2533-7602

Various quantitative PCR approaches have been utilized during the last years to provide information about the treatment efficacy and the risk of recurrent disease in haematological malignancies. Apart from the frequently used real-time PCR, cost-saving modified standard PCR methods may be applied as well. This report evaluates the utility of the end-point comparative duplex PCR. We have used this method for monitoring of 35 patients with either NHL or CLL and observed a good correlation between quantitative molecular results and clinical outcome. There was also an agreement between comparative duplex PCR and real-time PCR in patients who were monitored by both methods. We therefore believe that use of this technique should be strongly considered instead of simple qualitative detection in monitoring of therapeutic outcome in NHL or CLL patients.