DETECTION OF DISTRIBUTION OF AVIAN INFLUENZA H5N1 VIRUS BY IMMUNOHISTOCHEMISTRY, CHROMOGENIC IN SITU HYBRIDIZATION AND REAL-TIME PCR TECHNIQUES IN EXPERIMENTALLY INFECTED CHICKENS

• Published in: Southeast Asian journal of tropical medicine and public health Vol. 42; no. 2; pp. 303 - 310
• Main Authors: CHAMNANPOOD, Chanpen, SANGUANSERMSRI, Donruedee, PONGCHAROEN, Sutatip, SANGUANSERMSRI, Phanchana
• Format: Journal Article
• Language: English
• Published: Bangkok SEAMO, Regional Tropical Medicine and Public Health Network 01.03.2011; Central Coordinating Board, SEAMEO-TROPMED Project
• Subjects: Animals; Biological and medical sciences; Brain - virology; Bursa of Fabricius - virology; Chickens - virology; Digestive System - virology; General aspects; Heart - virology; Human viral diseases; Immunohistochemistry; In Situ Hybridization; Infectious diseases; Influenza A Virus, H5N1 Subtype - isolation & purification; Influenza in Birds - virology; Kidney - virology; Liver; Lung - virology; Medical sciences; Polymerase Chain Reaction; Spleen - virology; Thymus Gland - virology; Trachea - virology; Viral diseases; Viral diseases of the respiratory system and ent viral diseases; Immunohistochemistry; In situ; Infection; Avian influenza; Polymerase chain reaction; Influenzavirus A(H5N1); Anatomic pathology; Vertebrata; Viral disease; Distribution; Tropical medicine; Chicken; Technique; Aves; Molecular biology
• ISSN: 0125-1562

Ten specific pathogen free (SPF) chickens were inoculated intranasally with avian influenza virus subtype H5N1. Evaluation revealed distribution of the virus in twelve organs: liver, intestine, bursa, lung, trachea, thymus, heart, pancreas, brain, spleen, kidney, and esophagus. Immunohistochemistry (IHC), chromogenic in situ hybridization (CISH), and real-time polymerase chain reaction (PCR) were developed and compared for detection of the virus from the organs. The distribution of avian influenza H5N1 in chickens varied by animal and detecting technique. The heart, kidneys, intestines, lungs, and pancreas were positive with all three techniques, while the others varied by techique. The three techniques can be used to detect avian influenza effectively, but the pros and cons of each technique need to be determined. The decision of which technique to use depends on the objective of the examination, budget, type and quality of samples, laboratory facilities and technician skills.