p53 analysis of laryngeal cancer in exon 4 to 9

p53 gene mutations are a common genetic alteration in human cancer and codon 72/exon 4 polymorphism of the p53 gene has been implicated in cancer risk. Therefore in this study the p53 gene status of 32 shock-frozen tumor specimens from larynx carcinomas was analyzed by PCR and sequencing of exon 4 t...

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Published inAnticancer research Vol. 20; no. 4; p. 2613
Main Authors Gottschlich, S, Maune, S, Preugschat, J, Hoffmann, M, Werner, J A, Maass, J D, Görögh, T, Heidorn, K, Rudert, H
Format Journal Article
LanguageEnglish
Published Greece 01.07.2000
Subjects
Adult
Aged
Aged, 80 and over
Arginine - genetics
Codon
Exons
Female
Genes, p53
Humans
Laryngeal Neoplasms - genetics
Male
Middle Aged
Polymorphism, Genetic
Proline - genetics
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Summary:p53 gene mutations are a common genetic alteration in human cancer and codon 72/exon 4 polymorphism of the p53 gene has been implicated in cancer risk. Therefore in this study the p53 gene status of 32 shock-frozen tumor specimens from larynx carcinomas was analyzed by PCR and sequencing of exon 4 through 9. Four mutations (12.5%) in exon 5, 7, 8 and 9 were detected in the carcinoma specimen. Analysis of codon 72 revealed in eight cases a homozygosity for proline (CCC) and in 24 cases heterozygosity or homozygosity for arginine (CGC). The group with the proline/proline genotype had a median age 10.3 years lower than the remaining patients and included the only two non-smokers. Firstly, these results confirm the p53 mutational status of laryngeal cancer without any clinical correlation and secondly may suggest an oncogenic potential for the proline/proline genotype of codon 72 for laryngeal cancer as has already been assumed for lung cancer.
ISSN:0250-7005