A myeloid-lineage-specific enhancer upstream of the mouse myeloperoxidase (MPO) gene

The myeloperoxidase (MPO) gene is selectively expressed during haemopoiesis in the granulocytic lineage. Compared with the erythroid (beta-globin) and B-cell (immunoglobulin) lineages, little is known of the regulatory sequences and transcription factors involved in the regulation of genes specific...

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Bibliographic Details
Published inLeukemia Vol. 8; no. 5; p. 717
Main Authors Zhu, J, Bennett, C A, MacGregor, A D, Greaves, M F, Goodwin, G H, Ford, A M
Format Journal Article
LanguageEnglish
Published England 01.05.1994
Subjects
Animals
Base Sequence
Bone Marrow - pathology
Chromosome Mapping
Enhancer Elements, Genetic
Hematopoiesis - genetics
Leukemia, Myelomonocytic, Acute - genetics
Leukemia, Myelomonocytic, Acute - pathology
Mice
Molecular Sequence Data
Peroxidase - genetics
Transfection
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Summary:The myeloperoxidase (MPO) gene is selectively expressed during haemopoiesis in the granulocytic lineage. Compared with the erythroid (beta-globin) and B-cell (immunoglobulin) lineages, little is known of the regulatory sequences and transcription factors involved in the regulation of genes specific for granulopoiesis. We have approached this issue by identifying a strong enhancer for the murine MPO gene. A candidate enhancer region was mapped by the detection of a strong DNase I hypersensitive site, -3.4 to -3.2 kb upstream of the MPO gene. A 301 bp fragment encompassing the DNase I site was shown to have strong enhancer function in a transient assay following transfection of a reporter gene into a MPO-expressing cell (WEHI 3BD+), but was inactive in lymphoid cells. Analysis of sub-fragments revealed that the whole 301 bp fragment is required for maximal enhancer function.
ISSN:0887-6924