Amino acid synthesis loss in parasitoid wasps and other hymenopterans

• Published in: eLife Vol. 9
• Main Authors: Ye, Xinhai, Xiong, Shijiao, Teng, Ziwen, Yang, Yi, Wang, Jiale, Yu, Kaili, Wu, Huizi, Mei, Yang, Yan, Zhichao, Cheng, Sammy, Yin, Chuanlin, Wang, Fang, Yao, Hongwei, Fang, Qi, Song, Qisheng, Werren, John H, Ye, Gongyin, Li, Fei
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications, Ltd 19.10.2020
• Subjects: Genetics and Genomics; genetics; parasitoid wasps; genome sequencing; genomics; amino acid synthesis; trait loss; comparative genomics; Cotesia chilonis
• ISSN: 2050-084X
• DOI: 10.7554/eLife.59795

Insects utilize diverse food resources which can affect the evolution of their genomic repertoire, including leading to gene losses in different nutrient pathways. Here, we investigate gene loss in amino acid synthesis pathways, with special attention to hymenopterans and parasitoid wasps. Using comparative genomics, we find that synthesis capability for tryptophan, phenylalanine, tyrosine, and histidine was lost in holometabolous insects prior to hymenopteran divergence, while valine, leucine, and isoleucine were lost in the common ancestor of Hymenoptera. Subsequently, multiple loss events of lysine synthesis occurred independently in the Parasitoida and Aculeata. Experiments in the parasitoid confirm that it has lost the ability to synthesize eight amino acids. Our findings provide insights into amino acid synthesis evolution, and specifically can be used to inform the design of parasitoid artificial diets for pest control.