Establishment of lymphatic filarial specific IgG4 indirect ELISA detection method
To establish the lymphatic filarial specific IgG4 indirect ELISA detection method and develop the kits. ELISA and the developed specific IgG4 reagent was used to explore the best way for detecting filarial specific IgG4. Combined with the production process of commercialized enzyme immunoassay kit t...
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Published in | International journal of clinical and experimental medicine Vol. 8; no. 9; pp. 16496 - 16503 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
e-Century Publishing Corporation
01.01.2015
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Subjects | |
Online Access | Get full text |
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Summary: | To establish the lymphatic filarial specific IgG4 indirect ELISA detection method and develop the kits.
ELISA and the developed specific IgG4 reagent was used to explore the best way for detecting filarial specific IgG4. Combined with the production process of commercialized enzyme immunoassay kit to develop economical lymphatic filarial specific IgG4 test kit, and to explore the value of the kit in the laboratory.
We determined the most optimal detective antigen was Malay adult filarial antigen and the optimal concentration of coating antigen was 1.0 μg/ml. The appropriate serum dilution was 1:20 to 40 and the work titers of specific IgG4 agents was 1:800. We determined the optimal reaction time for substrates and developed a reproducible and stable detection kit with sensitive and specificity, which was easy to operate.
We successfully established the lymphatic filarial specific IgG4 indirect ELISA detection method and developed the kits with good reproducibility and stable result, which should be widely applied. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Equal contributors. |
ISSN: | 1940-5901 1940-5901 |