Protection by pyruvate and malate against glutamate-mediated neurotoxicity

Pyruvate and malate (P/M) increase the contribution of mitochondria to neuronal calcium homeostasis. We have now found that cortical neuronal cultures utilize pyruvate preferentially over glucose. The supply of pyruvate and malate protects hippocampal and cortical neurons against delayed cell death...

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Published inNeuroreport Vol. 9; no. 7; p. 1277
Main Authors Ruiz, F, Alvarez, G, Pereira, R, Hernández, M, Villalba, M, Cruz, F, Cerdán, S, Bogónez, E, Satrústegui, J
Format Journal Article
LanguageEnglish
Published England 11.05.1998
Subjects
Animals
Calcium - metabolism
Cell Survival - drug effects
Cells, Cultured
Cerebral Cortex - cytology
Embryo, Mammalian
Glutamic Acid - toxicity
Hippocampus - cytology
Kinetics
Malates - metabolism
Malates - pharmacology
Neurons - cytology
Neurons - drug effects
Neurons - metabolism
Neurotoxins - toxicity
Pyruvic Acid - metabolism
Pyruvic Acid - pharmacology
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Summary:Pyruvate and malate (P/M) increase the contribution of mitochondria to neuronal calcium homeostasis. We have now found that cortical neuronal cultures utilize pyruvate preferentially over glucose. The supply of pyruvate and malate protects hippocampal and cortical neurons against delayed cell death occurring 24 h after glutamate exposure. High [Ca2+]i levels attained during and after glutamate exposure were reduced when neurons were incubated in the presence of P/M. At the single cell level, this was reflected in a decrease in the number of neurons that respond to glutamate with high rises in [Ca2+]i. The results suggest that the ability to prevent large increases in [Ca2+]i may underlie the beneficial effects of pyruvate and malate during glutamate excitotoxicity.
ISSN:0959-4965