Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition

• Published in: International journal of clinical and experimental medicine Vol. 7; no. 3; pp. 540 - 548
• Main Authors: Li, Zhang-Hua, Liao, Wen, Zhao, Qiang, Huan, Tang, Feng, Pan, Wei, Xia, Yi, Yang, Shao, Ning-Sheng
• Format: Journal Article
• Language: English
• Published: United States e-Century Publishing Corporation 01.01.2014
• Subjects: Original; Mesenchymal stem cell; hypoxia; Core binding factor alpha l
• ISSN: 1940-5901; 1940-5901

To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia. The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR. After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P<0.05), especial the expression of Cbfa1 (P<0.05). After transfected with Cbfa1, cell morphology or growth cycle of MSCs was not significantly affected, but its osteogenic differentiation potential enhanced, particularly its osteogenic differentiation potential under hypoxia enhanced.