Comparison of three commercial media for direct identification and discrimination of Candida species in clinical specimens

• Published in: European journal of clinical microbiology & infectious diseases Vol. 16; no. 6; pp. 464 - 467
• Main Authors: FREYDIERE, A. M, BUCHAILLE, L, GILLE, Y
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.06.1997
• Subjects: Biological and medical sciences; Candida - classification; Candida - growth & development; Candida - isolation & purification; Candida albicans; Candida albicans - isolation & purification; Candida krusei; Candida tropicalis; Color; Culture Media; Evaluation Studies as Topic; Fundamental and applied biological sciences. Psychology; Humans; Microbiology; Mycological methods and techniques used in mycology; Mycological Typing Techniques; Mycology; Performance evaluation; Candidiasis; Mycosis; Identification; Method; Fungi; Infection; Candida; Isolate; Isolation; Fungi Imperfecti; Comparative study; Thallophyta
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/BF02471913

One hundred and ninety-two clinical specimens were used to compare the three chromogenic media Albicans ID, Candiselect, and CHROMagar Candida to a standard method using a Sabouraud-chloramphenicol agar medium and standard methods for identification of yeasts. The detection rates were 83.79, 83.24, 86.59 and 84.91% respectively. For the chromogenic media, the rates of direct identification (growth plus specific pigmentation) for Candida albicans were 56.50, 37.68 and 11.59% after 24 hours' incubation and 92.75, 91.30 and 88.57% after 72 hours' incubation respectively, with 100% specificity. Furthermore, CHROMagar Candida identified the five Candida tropicalis and the two Candida krusei strains detected after 48 hours' incubation.