Evaluation of fingerprinting methods for identification of Helicobacter pylori strains

• Published in: European journal of clinical microbiology & infectious diseases Vol. 10; no. 12; p. 1040
• Main Authors: Clayton, C L, Kleanthous, H, Dent, J C, McNulty, C A, Tabaqchali, S
• Format: Journal Article
• Language: English
• Published: Germany 01.12.1991
• Subjects: Animals; Bacterial Typing Techniques; Deoxyribonuclease HindIII - metabolism; DNA Fingerprinting - methods; DNA, Bacterial - analysis; Evaluation Studies as Topic; Helicobacter pylori - genetics; Helicobacter pylori - isolation & purification; Humans; Immunoblotting; Papio - microbiology; Polymorphism, Restriction Fragment Length; Restriction Mapping; Swine - microbiology
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/BF01984926

Variation amongst strains of Helicobacter pylori was examined by 35S-methionine-labelled protein SDS-PAGE (Radio-PAGE), immunoblot and DNA fingerprinting techniques. These methods allowed discrimination amongst isolates and showed total correlation. Pig and baboon gastric Helicobacter pylori-like organisms were found to be very similar to Helicobacter pylori by both Radio-PAGE and immunoblotting, whereas a Helicobacter mustelae isolate was markedly different. The HindIII restriction endonuclease digest patterns of Helicobacter pylori DNA illustrated the considerable genomic variation of this organism. The Radio-PAGE and immunoblot typing methods both gave precise identification of Helicobacter pylori strains, but Radio-PAGE was found to give higher resolution and represents a standardised universally applicable fingerprinting method for Helicobacter pylori.