Liquid Chromatography Tandem Mass/Mass Spectrometry for the Quantification of Fudosteine in Human Serum without Precolumn Derivatization

A quantitative analysis method for fudosteine in human serum by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) was established, which shows high sensitivity and selectivity. The mobile phase composition was 75% 20 mM acetic acid and 25% aceto...

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Bibliographic Details
Published inIranian journal of pharmaceutical research : IJPR Vol. 13; no. 2; pp. 441 - 447
Main Authors Fan, Peng-Cheng, Ren, Jun, Ma, Hui-Ping, Jing, Lin-Lin, Ma, Jun, Jia, Zheng-Ping
Format Journal Article
LanguageEnglish
Published Iran Shaheed Beheshti University of Medical Sciences 2014
Subjects
Original
HPLC-ESI/MS/MS
Human serum
Fudosteine
Pharmacokinetics
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Summary:A quantitative analysis method for fudosteine in human serum by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) was established, which shows high sensitivity and selectivity. The mobile phase composition was 75% 20 mM acetic acid and 25% acetonitril, which was pumped at a flow rate of 0.40 mL/min. The overall chromatographic run time was approximately 7 min. The autosampler was set with an injection volume of 10 μL. The calibration curve was linear in the concentration range of 0.1~15.0 µg/mL. The coefficient of determination (r) was greater than 0.9998. This method has been fully validated and shown to be specific, accurate and precise. The method was simple, rapid and the sample preparation was minimal. It was successfully applied to the analysis of healthy volunteer.
ISSN:1735-0328
1726-6890