Protective effect of paraoxonase 1 gene variant L55M in retinal vein occlusion

• Published in: Molecular vision Vol. 19; pp. 486 - xxx
• Main Authors: Ortak, Huseyin, Söğüt, Erkan, Ateş, Omer, Erkorkmaz, Unal, Benli, Ismail, Akbas, Ali, Demir, Selim, Ozyurt, Hüseyin
• Format: Journal Article
• Language: English
• Published: United States Molecular Vision 25.02.2013
• Subjects: Adult; Aged; Aged, 80 and over; Amino Acid Substitution - genetics; Aryldialkylphosphatase - genetics; Case-Control Studies; Ethnic Groups - genetics; Female; Genetic Variation; Humans; Male; Middle Aged; Polymorphism, Single Nucleotide; Prospective Studies; Retinal Vein Occlusion - enzymology; Retinal Vein Occlusion - etiology; Retinal Vein Occlusion - genetics; Risk Factors; Turkey - ethnology; Turkey
• ISSN: 1090-0535; 1090-0535

To determine if the paraoxonase 1 L55M and paraoxonase 1 Q192R gene polymorphisms have an effect on the risk of having a retinal vein occlusion (RVO). This case-control prospective study included 120 patients with RVO and 84 control subjects. All subjects were screened for age, gender, hypertension, diabetes, body mass index, fibrinogen, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, total cholesterol, and very low-density lipoprotein. Subjects were also questioned about their smoking habits. Genomic DNA was extracted from peripheral leukocytes from EDTA anticoagulated blood. Genotyping of the paraoxonase 1 L55M and paraoxonase 1 Q192R polymorphisms was performed using real-time PCR. The frequency of the paraoxonase 1 (PON1) 55 LL genotype was significantly lower in patients with RVO than in the control subjects (28% versus 55%; p = 0.005). Logistic regression analyses were also conducted. After adjusting for gender, diabetes, hypertension, plasma fibrinogen levels, and high-density lipoprotein cholesterol, the lower LL genotype was found to be an independent predictor of RVO (β = 1.755; odds ratio = 5.783; p < 0.001; 95% confidence interval = 2.579-12.967). Subjects with a lower frequency PON1 55 LL genotype had a higher risk of RVO. These results indicate that paraoxonase gene polymorphisms may be a possible risk factor for RVO. We suggest that the LL genotype may have a protective role in the pathogenesis of RVO in the Turkish population.