Metabolism of vertebrate amino sugars with N-glycolyl groups: resistance of α2-8-linked N-glycolylneuraminic acid to enzymatic cleavage

• Published in: The Journal of biological chemistry Vol. 287; no. 34; pp. 28917 - 28931
• Main Authors: Davies, Leela R L, Pearce, Oliver M T, Tessier, Matthew B, Assar, Siavash, Smutova, Victoria, Pajunen, Maria, Sumida, Mizuki, Sato, Chihiro, Kitajima, Ken, Finne, Jukka, Gagneux, Pascal, Pshezhetsky, Alexey, Woods, Robert, Varki, Ajit
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 17.08.2012
• Subjects: Amino Sugars - chemistry; Amino Sugars - metabolism; Animals; Bacteria - chemistry; Bacteria - metabolism; Brain - metabolism; Brain Chemistry - physiology; Carbohydrate Conformation; Cattle; Dolphins; Elephants; Evolution, Molecular; Glycobiology and Extracellular Matrices; Hydrolysis; Mice; N-Acetylneuraminic Acid; Nerve Tissue Proteins - chemistry; Nerve Tissue Proteins - metabolism; Neuraminic Acids - chemistry; Neuraminic Acids - metabolism; Neuraminidase - chemistry; Neuraminidase - metabolism; Pan troglodytes; Rats; Species Specificity; Swine
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M112.365056

The sialic acid (Sia) N-acetylneuraminic acid (Neu5Ac) and its hydroxylated derivative N-glycolylneuraminic acid (Neu5Gc) differ by one oxygen atom. CMP-Neu5Gc is synthesized from CMP-Neu5Ac, with Neu5Gc representing a highly variable fraction of total Sias in various tissues and among different species. The exception may be the brain, where Neu5Ac is abundant and Neu5Gc is reported to be rare. Here, we confirm this unusual pattern and its evolutionary conservation in additional samples from various species, concluding that brain Neu5Gc expression has been maintained at extremely low levels over hundreds of millions of years of vertebrate evolution. Most explanations for this pattern do not require maintaining neural Neu5Gc at such low levels. We hypothesized that resistance of α2-8-linked Neu5Gc to vertebrate sialidases is the detrimental effect requiring the relative absence of Neu5Gc from brain. This linkage is prominent in polysialic acid (polySia), a molecule with critical roles in vertebrate neural development. We show that Neu5Gc is incorporated into neural polySia and does not cause in vitro toxicity. Synthetic polymers of Neu5Ac and Neu5Gc showed that mammalian and bacterial sialidases are much less able to hydrolyze α2-8-linked Neu5Gc at the nonreducing terminus. Notably, this difference was not seen with acid-catalyzed hydrolysis of polySias. Molecular dynamics modeling indicates that differences in the three-dimensional conformation of terminal saccharides may partly explain reduced enzymatic activity. In keeping with this, polymers of N-propionylneuraminic acid are sensitive to sialidases. Resistance of Neu5Gc-containing polySia to sialidases provides a potential explanation for the rarity of Neu5Gc in the vertebrate brain.