Forskolin-insensitive adenylate cyclase in cultured cells of Choristoneura fumiferana (Insecta)

Adenylate cyclase from a spruce budworm cell line, IBRI-Cfl, is activated by octopamine (Ka = 50 microM), guanine nucleotides and sodium fluoride but not by forskolin. In addition, forskolin does not potentiate the octopamine-sensitive response. To our knowledge, this is the first published report o...

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Published inBiochemical and biophysical research communications Vol. 145; no. 3; pp. 1192 - 1197
Main Authors GOLE, J. W. D, ORR, G. L, DOWNER, R. G. H
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier 30.06.1987
Subjects
1-Methyl-3-isobutylxanthine - pharmacology
adenylate cyclase
Adenylyl Cyclases - metabolism
Animals
Applied sciences
Cell Line
Choristoneura fumiferana
Colforsin - pharmacology
Exact sciences and technology
forskolin
Guanosine Triphosphate - pharmacology
Guanylyl Imidodiphosphate - pharmacology
Kinetics
Lepidoptera - enzymology
Octopamine - pharmacology
Other techniques and industries
Sodium Fluoride - pharmacology
Tortricidae
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Summary:Adenylate cyclase from a spruce budworm cell line, IBRI-Cfl, is activated by octopamine (Ka = 50 microM), guanine nucleotides and sodium fluoride but not by forskolin. In addition, forskolin does not potentiate the octopamine-sensitive response. To our knowledge, this is the first published report of a hormone-sensitive adenylate cyclase, with a functional guanine nucleotide regulatory protein, that is insensitive to forskolin both in intact cells and washed membrane preparations.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(87)91563-4