Forskolin-insensitive adenylate cyclase in cultured cells of Choristoneura fumiferana (Insecta)
Adenylate cyclase from a spruce budworm cell line, IBRI-Cfl, is activated by octopamine (Ka = 50 microM), guanine nucleotides and sodium fluoride but not by forskolin. In addition, forskolin does not potentiate the octopamine-sensitive response. To our knowledge, this is the first published report o...
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Published in | Biochemical and biophysical research communications Vol. 145; no. 3; pp. 1192 - 1197 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier
30.06.1987
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Subjects | |
Online Access | Get full text |
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Summary: | Adenylate cyclase from a spruce budworm cell line, IBRI-Cfl, is activated by octopamine (Ka = 50 microM), guanine nucleotides and sodium fluoride but not by forskolin. In addition, forskolin does not potentiate the octopamine-sensitive response. To our knowledge, this is the first published report of a hormone-sensitive adenylate cyclase, with a functional guanine nucleotide regulatory protein, that is insensitive to forskolin both in intact cells and washed membrane preparations. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/0006-291X(87)91563-4 |