Microbial models of mammalian metabolism. Furosemide glucoside formation using the fungus Cunninghamella elegans

The diuretic furosemide (Lasix) was metabolized by the fungus Cunninghamella elegans (ATCC 36112) to the phase II conjugate, furosemide acyl glucoside. This metabolite was isolated following semipreparative scale incubations of C. elegans involving glucose nutrient dosing, and was characterized by N...

Full description

Saved in:
Bibliographic Details
Published inDrug metabolism and disposition Vol. 21; no. 2; p. 259
Main Authors Hezari, M, Davis, P J
Format Journal Article
LanguageEnglish
Published United States 01.03.1993
Subjects
alpha-Glucosidases - pharmacology
beta-Glucosidase - pharmacology
Chromatography, High Pressure Liquid
Fermentation
Furosemide - analysis
Furosemide - metabolism
Gas Chromatography-Mass Spectrometry
Glucose - metabolism
Glucosides - metabolism
Glucuronidase - pharmacology
Hydrolysis
Magnetic Resonance Spectroscopy
Models, Biological
Mucorales - metabolism
Spectrometry, Fluorescence
Spectrometry, Mass, Fast Atom Bombardment
Spectrophotometry, Ultraviolet
Online AccessGet more information

Cover

More Information
Summary:The diuretic furosemide (Lasix) was metabolized by the fungus Cunninghamella elegans (ATCC 36112) to the phase II conjugate, furosemide acyl glucoside. This metabolite was isolated following semipreparative scale incubations of C. elegans involving glucose nutrient dosing, and was characterized by NMR spectroscopy (1H and 1H/1H correlated), MS (FAB), UV, HPLC with fluorescence detection, and enzymatic treatments. The aglycone fragment of the conjugate was characterized as furosemide by treatment of the metabolite with sodium hydroxide, whereas the sugar part was identified as glucose by cleavage of the conjugate, derivatization of the released sugar, and GC/MS analysis.
ISSN:0090-9556