L1CAM is involved in lymph node metastasis via ERK1/2 signaling in colorectal cancer

• Published in: American journal of translational research Vol. 12; no. 3; pp. 837 - 846
• Main Authors: Fang, Qing-Xia, Zheng, Xiao-Chun, Zhao, Hua-Jun
• Format: Journal Article
• Language: English
• Published: United States e-Century Publishing Corporation 01.01.2020
• Subjects: Original; ERK1/2; colorectal cancer; L1; lymph node metastatic
• ISSN: 1943-8141; 1943-8141

L1-cell adhesion molecule (L1CAM, L1) belongs to the immunoglobulin superfamily and was originally found to play a role in nerve cells. Recently, the expression and prognostic value of L1 has been established in several cancers, including colorectal cancer (CRC). However, its association with lymph node metastasis in CRC and the mechanisms underlying its effects remain unclear. In this study, we evaluated the L1 transcript levels in CRC (n=12) and normal intestinal tissues (n=15) by qRT-PCR. Western blotting was used to evaluate L1 and pERK1/2 expression levels. Immunohistochemistry was performed to evaluate the relationship between L1 and pERK1/2 in CRC tissues with different levels of differentiation. The mRNA expression levels in CRC tissues were significantly higher compared to normal intestinal tissues. Western blotting demonstrated that both L1 and pERK1/2 levels were higher in CRC than in normal tissues. Immunohistochemistry confirmed that L1 and pERK1/2 levels in adenomas with lymph node metastasis were significantly higher than in poorly and well-differentiated adenomas, indicating that L1 and pERK1/2 levels correlated with CRC lymph node metastasis. In conclusion, L1 and pERK1/2 were significantly up-regulated in CRC tissues and lymph node metastasis may occur via the L1CAM-mediated ERK pathway in CRC.