Use of ELISA for in vitro potency test of rabies vaccines for animal use

• Published in: Biologicals Vol. 24; no. 2; pp. 95 - 101
• Main Authors: GAMOH, K, SENDA, M, ITOH, O, MURAMATSU, M, HIRAYAMA, N, KOIKE, R, ENDOH, Y. S, MINAMOTO, N
• Format: Journal Article
• Language: English
• Published: Kent Elsevier Science 01.06.1996
• Subjects: Animals; Biological and medical sciences; Blotting, Western; Chromatography, High Pressure Liquid; Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Guinea Pigs; Mice; Microbiology; Rabies Vaccines; rabies virus; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Virology; Immunoprotection; Vaccine; Enzyme immunoassay; In vitro; Rabies virus; Infection; Virus; Investigation method; Rhabdoviridae; Efficiency; Viral disease; Rabies; Lyssavirus; Veterinary; ELISA assay; Inactivated strain
• ISSN: 1045-1056; 1095-8320
• DOI: 10.1006/biol.1996.0012

The use of ELISA for the potency test of inactivated rabies vaccines for animal use was evaluated. Sandwich ELISA was developed, using monoclonal antibodies which enabled to recognize the glycoprotein (G-protein) of rabies virus and showed a protective effect in mice. Soluble G-protein which displays an ELISA activity but a low immunogenicity was removed by gel filtration before ELISA was carried out. The ELISA titres of the first fraction of vaccines separated by gel filtration were correlated with the neutralizing antibody titres of vaccinated guinea-pigs. Our results suggest that the ELISA titres of the first fraction of vaccines separated by gel filtration reflected the protective G-protein contents for the in vitro potency test of rabies vaccines compared with the current in vivo test.