Possible involvement of calpain in the growth of estrogen receptor positive breast cancer cells

Calpain (Ca2(+)-activated neutral protease, EC 3.4.22.17) has been reported to hydrolyze the estrogen receptor (ER). However, there has been no report available regarding the role of calpain in the growth of breast cancer cells. To investigate the role of calpain in the growth of various breast canc...

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Bibliographic Details
Published inAnticancer research Vol. 16; no. 2; p. 773
Main Authors Shiba, E, Kim, S, Fujitani, M, Kambayashi, J I, Kawamura, I, Tsujimoto, S, Shimomura, K, Tanji, Y, Taguchi, T, Kimoto, Y, Izukura, M, Takai, S I
Format Journal Article
LanguageEnglish
Published Greece 01.03.1996
Subjects
Animals
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Calpain - antagonists & inhibitors
Calpain - physiology
Cell Division
Dipeptides - metabolism
Dipeptides - pharmacology
Female
Humans
Rats
Receptors, Estrogen - metabolism
Tumor Cells, Cultured
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Summary:Calpain (Ca2(+)-activated neutral protease, EC 3.4.22.17) has been reported to hydrolyze the estrogen receptor (ER). However, there has been no report available regarding the role of calpain in the growth of breast cancer cells. To investigate the role of calpain in the growth of various breast cancer cell lines, we employed a synthetic peptide, calpeptin, which is a cell permeable specific inhibitor of calpain. Calpeptin inhibited the cell growth of ER positive breast cancer cells, such as MCF-7, T-47D, and ZR-75-1 in a dose dependent manner in the presence of E2. However, the growth of ER negative breast cancer cells, MDA-MB-231, was not inhibited by calpeptin. It is suggested that calpain plays an important role in the growth of ER positive breast cancer cells.
ISSN:0250-7005