Genetic requirements for hyper-recombination by very short patch mismatch repair: involvement of Escherichia coli DNA polymerase I

It has been established that very short patch (VSP) mismatch repair, depending in Escherichia coli on MutL, MutS and Dcm functions, is responsible for the hyper-recombinogenic effect of a class of genetic markers. We show that VSP repair requires the presence of the complete DNA polymerase I enzyme....

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Bibliographic Details
Published inMolecular & general genetics Vol. 217; no. 2-3; p. 254
Main Authors Dzidic, S, Radman, M
Format Journal Article
LanguageEnglish
Published Germany 01.06.1989
Subjects
Base Composition
Base Sequence
Crosses, Genetic
DNA Polymerase I - metabolism
DNA Repair
Escherichia coli - genetics
Escherichia coli - metabolism
Genetic Markers
Mutation
Recombination, Genetic
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Summary:It has been established that very short patch (VSP) mismatch repair, depending in Escherichia coli on MutL, MutS and Dcm functions, is responsible for the hyper-recombinogenic effect of a class of genetic markers. We show that VSP repair requires the presence of the complete DNA polymerase I enzyme. The absence of endonuclease activities involved in the repair of base-loss sites, Nth, Nfo and Xth, does not affect VSP repair. Implications for the mechanism of the VSP repair are discussed.
ISSN:0026-8925
DOI:10.1007/BF02464889