Hepatoprotective effect of an aqueous extract of the leaves of Acalypha wilkesiana 'Godseffiana' Muell Arg (Euphorbiaceae) against carbon tetrachloride induced liver injury in rats

• Published in: EXCLI journal Vol. 10; pp. 280 - 289
• Main Authors: Ikewuchi, Jude C, Uwakwe, Augustine A, Onyeike, Eugene N, Ikewuchi, Catherine C
• Format: Journal Article
• Language: English
• Published: Germany Leibniz Research Centre for Working Environment and Human Factors 01.01.2011
• Subjects: Acalypha wilkesiana 'Godseffiana' Muell Arg (Euphorbiaceae); histopathology; carbon tetrachloride; hepatospecific markers
• ISSN: 1611-2156; 1611-2156

The potential of aqueous extract of the leaves of , to protect against carbon tetrachloride induced liver damage was investigated in Wistar albino rats. The carbon tetrachloride was prepared 1:5 (v:v) in olive oil, and administered subcutaneously at 1 mL/kg body weight. The extract was administered to both normal and carbon tetrachloride treated rats at 100, 200 and 300 mg/kg. On fractionation and gas chromatographic analysis of the crude aqueous extract, thirty nine known alkaloids were detected, consisting mainly of akuamidine (69.027 %), voacangine (26.226 %), echitamine (1.974 %), echitamidine (0.599 %), lupanine (0.521 %) and augustamine (0.278 %). Compared to test control, the treatment dose dependently produced significantly lower ( <0.05) alkaline phosphatase, aspartate and alanine transaminase activities. Histopathological studies on the liver sections showed that pre-treatment with the extract protected against carbon tetrachloride induced fatty degeneration of hepatocytes, thus, confirming the results of the biochemical studies. The above results imply that treatment with the plant extract protects the liver against carbon tetrachloride induced hepatotoxicity, therefore, justifying the use of in African traditional health care for the management of liver problems.