Detection of p53 mutations in B cell non-Hodgkin's lymphoma cell lines

• Published in: Leukemia Vol. 9; no. 4; p. 650
• Main Authors: Li, C C, O'Connell, C D, Beckwith, M, Longo, D L
• Format: Journal Article
• Language: English
• Published: England 01.04.1995
• Subjects: B-Lymphocytes; Base Sequence; Cell Line; DNA Primers - chemistry; Genes, p53; Humans; Lymphoma, B-Cell - genetics; Molecular Sequence Data; Mutation; Polymorphism, Single-Stranded Conformational
• ISSN: 0887-6924

The p53 tumor suppressor gene is frequently mutated within its evolutionarily conserved regions in a number of human cancers. Previous reports demonstrated mutations of this gene in both Burkitt's lymphoma and B cell chronic lymphocytic leukemia. However, dissimilar results were obtained in non-Hodgkin's lymphoma (NHL). In one study, no mutation was detected in 43 NHL tissues. A second study reported p53 mutations in eight (all with advanced stage disease) out of 48 tissues obtained from Japanese NHL patients. Using both immunoblotting and radio-immunoprecipitation, we detected mutant p53 proteins in nine out of 10 B cell lines established from NHL tissues. The mutations were confirmed by reverse transcription polymerase chain reaction-mediated single-strand conformational polymorphism (RT-PCR-SSCP) analysis in eight cell lines. The high frequency of p53 mutation in NHL B cell lines and the relatively low frequency of p53 mutations in fresh lymphoma tissue suggests that p53 gene alteration may play a role in lymphomagenesis and/or disease progression in a subset of B cell lymphomas and that the p53 mutation conveys a proliferative advantage on lymphoma cells that permits their in vitro growth.