HIF-1α change in serum and callus during fracture healing in ovariectomized mice

• Published in: International journal of clinical and experimental pathology Vol. 8; no. 1; pp. 117 - 126
• Main Authors: Li, Wenliang, Wang, Kejie, Liu, Zhiwei, Ding, Wenge
• Format: Journal Article
• Language: English
• Published: United States e-Century Publishing Corporation 2015
• Subjects: Animals; Bone Density; Bony Callus - metabolism; Enzyme-Linked Immunosorbent Assay; Female; Femoral Fractures - metabolism; Femoral Fractures - pathology; Fracture Healing - physiology; Humans; Hypoxia-Inducible Factor 1, alpha Subunit - analysis; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Immunohistochemistry; Mice; Mice, Inbred C57BL; Neovascularization, Physiologic - physiology; Original; Osteoporosis, Postmenopausal - metabolism; Ovariectomy; X-Ray Microtomography; HIF-1α; osteoporosis; fracture healing; ovariectomy
• ISSN: 1936-2625

The purpose was to detect the effects of ovariectomy (OVX) on femoral fracture healing through different angiogenesis and HIF-1α expression in mice. Thirty-six young female C57 mice were randomized into two groups: OVX and age-matched intact control (CON). The femoral fracture was generated at 3 weeks after OVX or CON. At 2 or 4 weeks after fracture, the femoral fracture area was evaluated healing status by bone mineral density (BMD), callus formation and mineralization and neovascularization in callus, biomechanical analysis, and HIF-1α tests. OVX mice showed lower BMD as compared with CON mice. Callus geometric microstructural parameters of the femora in OVX mice were significantly lower than CON mice. OVX induced significant changes of biomechanical parameters in the femoral fracture healing area. The callus forming, callus neovascularization and HIF-1α tests in OVX mice were significantly lower than in CON mice. HIF-1α results have the positive proportion with osteoporotic fracture healing.