LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples

• Published in: Iranian journal of veterinary research Vol. 18; no. 2; pp. 134 - 137
• Main Authors: Deb, R, Sengar, G S, Singh, U, Kumar, S, Raja, T V, Alex, R, Alyethodi, R R, Prakash, B
• Format: Journal Article
• Language: English
• Published: Iran School of Veterinary Medicine, University of Shiraz 01.01.2017
• Subjects: Short Paper; Cow; Goat; LAMP assay; Meat; Milk
• ISSN: 1728-1997; 2252-0589
• DOI: 10.22099/ijvr.2017.4099

Animal species detection is one of the crucial steps for consumer's food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Green I dyes or by detecting the typical ladder pattern on gel electrophoresis. This test can detect up to minimum 5% level of cow components admixed in goat milk/meat samples and can be completed within 1 h 40 min starting from DNA extraction from milk/meat samples and can be performed in a water bath. Developed LAMP methodology is simple; rapid and sensitive techniques that can detect adulterant like cow components in goat milk/meat are more accurate than other existing DNA based technologies.