Reisolation of Ruminobacter parvum

• Published in: Antonie van Leeuwenhoek Vol. 51; no. 2; p. 129
• Main Authors: Lankhorst, A, Prins, R A
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.1985
• Subjects: Animals; Bacteria, Anaerobic - growth & development; Bacteria, Anaerobic - isolation & purification; Bacteria, Anaerobic - metabolism; Cattle; Cellulose - metabolism; Culture Media; DNA, Bacterial - analysis; Ethanol - metabolism; Fatty Acids - metabolism; Fatty Acids, Volatile - metabolism; Fermentation; Gram-Negative Bacteria - analysis; Gram-Negative Bacteria - growth & development; Gram-Negative Bacteria - isolation & purification; Gram-Negative Bacteria - metabolism; Hordeum; Hydrogen-Ion Concentration; Oxygen - pharmacology; Rumen - microbiology; Temperature
• ISSN: 0003-6072
• DOI: 10.1007/BF02310006

A cellulolytic gram-negative ovoid motile rod (strain GS III) was isolated from an in vitro incubation of ground barley straw in rumen fluid. The anaerobic, non-sporulating, mesophilic organism strongly answered the original description of Ruminobacter parvum (Kaars Sijpesteijn, 1948). The strain GS III fermented pyruvate, D-arabinose, D-xylose, cellobiose, sucrose, maltose, cellulose, dextrin, xylan and pectin. Products from cellobiose were D-lactate, ethanol, acetate, hydrogen and carbon dioxide. A heat-resistant factor in yeast extract that was not a B-vitamin, a metal or one of the volatile fatty acids was required for growth. The mol % G + C was 51.9. The organism was lost after prolonged subculture.