Rapid and sensitive detection of Campylobacter spp. from chicken using the polymerase chain reaction

• Published in: Zentralblatt für Bakteriologie Vol. 285; no. 4; pp. 480 - 485
• Main Authors: OYOFO, B. A, ABD EL SALAM, S. M, CHURILLA, A. M, WASFY, M. O
• Format: Journal Article
• Language: English
• Published: Jena Urban & Fischer 01.04.1997
• Subjects: Animal bacterial diseases; Animals; Bacterial diseases; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Campylobacter; Campylobacter - genetics; Campylobacter - isolation & purification; Chickens - microbiology; Cloaca - microbiology; DNA, Bacterial - analysis; Flagellin - genetics; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; Infectious diseases; Medical sciences; Microbiology; Polymerase Chain Reaction; Sensitivity and Specificity; Campylobacteraceae; Flagellin; Infection; Polymerase chain reaction; Vertebrata; DNA; Bacteriosis; Bacteria; Chicken; Diagnosis; Aves; Veterinary; Detection; Biological contamination; Campylobacter
• ISSN: 0934-8840
• DOI: 10.1016/S0934-8840(97)80108-9

The polymerase chain reaction (PCR) using a target region in the flaA gene of C. coli VC167 flagellin was used to detect Campylobacter spp. in chicken without an enrichment culture. DNA extracted from 79 cloacal swabs from broiler chickens gave an amplification signal in the 450-bp region upon PCR. DNA extracted from 9 enteric and 6 non-enteric organisms included in the assay as negative controls failed to hybridize with the probe. Direct plating of all cloacal specimens on Campylobacter blood agar plates did not yield any growth. The PCR assay was sensitive enough to detect between 35-120 bacteria per PCR and thus provide a basis for detecting Campylobacter spp. in poultry.