Aptamer‐Functionalized Magnetic Ti3C2 Based Nanoplatform for Simultaneous Enrichment and Detection of Exosomes

• Published in: Small (Weinheim an der Bergstrasse, Germany) Vol. 20; no. 44; pp. e2402434 - n/a
• Main Authors: Cui, Hongyuan, Zheng, Tianfang, Qian, Nana, Fu, Xueqi, Li, Aijun, Xing, Shu, Wang, Xiao‐Feng
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley Subscription Services, Inc 01.11.2024
• Subjects: aptamers; Biomarkers; Composite materials; detection; Effectiveness; Enrichment; exosome; Iron oxides; Magnetic separation; MXenes; Ti3C2; Tumors
• ISSN: 1613-6810; 1613-6829; 1613-6829
• DOI: 10.1002/smll.202402434

Exosomes are nanovesicles secreted by cells, which play a crucial role in various pathological processes. Exosomes have shown great promise as tumor biomarkers because of the abundant secretion during tumor formation. The development of a convenient, efficient, and cost‐effective method for simultaneously enriching and detecting exosomes is of utmost importance for both basic research and clinical applications. In this study, an aptamer‐functionalized magnetic Ti3C2 composite material (Fe3O4@Ti3C2@PEI@DSP@aptamer@FAM‐ssDNA) is prepared for the simultaneous enrichment and detection of exosomes. CD63 aptamers are utilized to recognize and capture the exosomes, followed by magnetic separation. The exosomes are then released by cleaving the disulfide bonds of DSP. Compared to traditional methods, Fe3O4@Ti3C2@PEI@DSP@aptamer@FAM‐ssDNA exhibited superior efficiency in enriching exosomes while preserving their structural and functional integrity. Detection of exosome concentration is achieved through the fluorescence quenching of Ti3C2 and the competitive binding between the exosomes and a fluorescently labeled probe. This method exhibited a low detection limit of 4.21 × 104 particles mL−1, a number that is comparable to the state‐of‐the‐art method in the detection of exosomes. The present study demonstrates a method of simultaneous enrichment and detection of exosomes with a high sensitivity, accuracy, specificity, and cost‐effectiveness providing significant potential for clinical research and diagnosis. A magnetic Ti3C2 composite material Fe3O4@Ti3C2@PEI@DSP@aptamer@FAM‐ssDNA is synthesized for simultaneous enrichment and detection of exosomes. Compared to traditional methods, Fe3O4@Ti3C2@PEI@DSP@aptamer@FAM‐ssDNA exhibits higher enrichment efficiency and preserves the structure and functionality of captured exosomes. Additionally, Fe3O4@Ti3C2@PEI@DSP@aptamer@FAM‐ssDNA enables quantitative detection of the enriched exosomes with a low detection limit of 4.21 × 104 particles mL−1 and possesses high sensitivity, accuracy, specificity, and low cost.