One‐Pot Synthesis of Nanoflower‐Like Zn2SnS4 as Nanozymes for Highly Sensitive Electrochemical Detection of H2O2 Released by Living Cells

• Published in: Chemistry : a European journal Vol. 30; no. 32; pp. e202400700 - n/a
• Main Authors: Wen, Xia, Ni, Jiancong, Zeng, Shunmu, Song, Zhiping, Qiu, Weiwei
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley Subscription Services, Inc 06.06.2024
• Subjects: Bimetals; Cancer therapies; Cell differentiation; Cells (biology); Chemical sensors; Electrochemical analysis; Electrochemistry; Hydrogen peroxide; Leukemia; Living cells; Low concentrations; Macrophages; Metal ions; Microenvironments; Monitoring; Nanoflower-like bimetallic sulfides; Nanoparticles; Nanozyme; One-pot hydrothermal; Sensors
• ISSN: 0947-6539; 1521-3765
• DOI: 10.1002/chem.202400700

The sensitive and reliable nanozyme‐based sensor enables the detection of low concentrations of H2O2 in biological microenvironments, it has potential applications as an in‐situ monitoring platform for cellular H2O2 release. The uniformly dispersed bimetallic sulfide (Zn2SnS4) nanoflowers were synthesized via a one‐pot hydrothermal method and the two kinds of metal ions can serve as morphology and structure directing agents for each other in the synthetic process. The nanoparticles were utilized as nanozyme materials to fabricate a novel electrochemical sensor, and it exhibits a distinct electrochemical response towards H2O2 with excellent stability and detection capability (with a minimum detection limit of 1.79 nM (S/N=3)), the excellent characteristics facilitate the precise detection of low concentrations of H2O2 in biological microenvironments. Use the macrophages differentiated from leukemia THP‐1 cells as a representative sensing model, the sensor was successfully utilized for real‐time monitoring of the release of H2O2 induced by living cells, which has significant potential applications in clinical diagnosis and cancer treatment. Zn2SnS4 nanoflowers with high uniformity and a large surface area were synthesized using a facile one‐pot hydrothermal method without the use of any template precursors. Utilizing macrophages differentiated from leukemia THP‐1 cells as a representative sensing model, an electrochemical sensor based on the Zn2SnS4 nanoflowers was successfully fabricated for real‐time monitoring of H2O2 release from living cells induced by LPS.