A relationship between RP4 plasmid acquisition and phenotypic changes in Pseudomonas fluorescens R2fN

The physiological behaviour of Pseudomonas fluorescens strain R2fN was compared to that of transconjugants [R2fN(RP4)], and two aggregation phenotypes were identified (Agr- and Agr+). Agr+ phenotype is characterized by the appearance of macroscopic aggregates when cells are growing in liquid media....

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Published inAntonie van Leeuwenhoek Vol. 79; no. 2; pp. 173 - 178
Main Authors Zgoda, A, Chablain, P, Mater, D, Truffaut, N, Barbotin, J N, Thomas, D
Format Journal Article
LanguageEnglish
Published Netherlands Springer Nature B.V 01.06.2001
Subjects
Bacteria
Bacterial Adhesion
Conjugation, Genetic
Culture Media
Hydrocarbons
Hydrophobic and Hydrophilic Interactions
Microbiology
Microscopy, Electron, Scanning
Phenotype
Plasmids
Pseudomonas fluorescens - classification
Pseudomonas fluorescens - genetics
Pseudomonas fluorescens - physiology
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Summary:The physiological behaviour of Pseudomonas fluorescens strain R2fN was compared to that of transconjugants [R2fN(RP4)], and two aggregation phenotypes were identified (Agr- and Agr+). Agr+ phenotype is characterized by the appearance of macroscopic aggregates when cells are growing in liquid media. Transconjugants exhibited Agr+ phenotype whereas wild type strain represented Agr-. Evidence is presented to support correlation between Agr+ phenotype acquisition and the presence of the broad-host range plasmid RP4 in strain R2fN. In addition, according to bacterial adherence to hydrocarbon test the transconjugant cells appeared to be very hydrophilic whereas wild type R2fN cells were hydrophobic.
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ISSN:0003-6072
1572-9699
DOI:10.1023/A:1010262817895