Genome Analyses of Two Blueberry Pathogens: Diaporthe amygdali CAA958 and Diaporthe eres CBS 160.32

• Published in: Journal of fungi (Basel) Vol. 8; no. 8; p. 804
• Main Authors: Hilário, Sandra, Gonçalves, Micael F M, Fidalgo, Cátia, Tacão, Marta, Alves, Artur
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.08.2022; MDPI
• Subjects: Comparative analysis; Diaporthe; Diaporthe amygdali; Diaporthe eres; Dieback; Enzymes; Gene clusters; Genes; Genomes; Host plants; Metabolites; Pathogenicity; Pathogens; Peptides; Phytotoxins; Proteins; Secretome; Software; Transfer RNA; Virulence; United States > US; Portugal; Germany; whole genome sequencing; effectors; Fusicoccin A; virulence factors; CAZymes; Diaporthe
• ISSN: 2309-608X
• DOI: 10.3390/jof8080804

The genus includes pathogenic species distributed worldwide and affecting a wide variety of hosts. and have been found to cause cankers, dieback, or twig blights on economically important crops such as soybean, almond, grapevine, and blueberry. Despite their importance as plant pathogens, the strategies of species of to infect host plants are poorly explored. To provide a genomic basis of pathogenicity, the genomes of CAA958 and CBS 160.32 were sequenced and analyzed. Cellular transporters involved in the transport of toxins, ions, sugars, effectors, and genes implicated in pathogenicity were detected in both genomes. Hydrolases and oxidoreductases were the most prevalent carbohydrate-active enzymes (CAZymes). However, analyses of the secreted proteins revealed that the secretome of CBS 160.32 is represented by 5.4% of CAZymes, whereas the secreted CAZymes repertoire of CAA958 represents 29.1% of all secretomes. Biosynthetic gene clusters (BGCs) encoding compounds related to phytotoxins and mycotoxins were detected in and genomes. The core gene clusters of the phytotoxin Fusicoccin A in are reported here through a genome-scale assembly. Comparative analyses of the genomes from 11 species revealed an average of 874 CAZymes, 101 secondary metabolite BGCs, 1640 secreted proteins per species, and genome sizes ranging from 51.5 to 63.6 Mbp. This study offers insights into the overall features and characteristics of genomes. Our findings enrich the knowledge about and , which will facilitate further research into the pathogenicity mechanisms of these species.