The effect of an IgM plasmacytoma (TEPC-183) on the primary immune response of BALB/c mice
Mice bearing plasmacytoma TEPC-183 IgM(k) have suppressed immune responses (IR) to both T-dependent and T-independent antigens, i.e. 2-4-dinitrophenyl (DNP) and the pneumococcal polysaccharides types I, III and XIV (SSS-I, SSS-III and SSS-XIV). Although these polysaccharides differ structurally and...
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Published in | Immunology Vol. 34; no. 1; pp. 1 - 8 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
01.01.1978
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Subjects | |
Online Access | Get full text |
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Summary: | Mice bearing plasmacytoma TEPC-183 IgM(k) have suppressed immune responses (IR) to both T-dependent and T-independent antigens, i.e. 2-4-dinitrophenyl (DNP) and the pneumococcal polysaccharides types I, III and XIV (SSS-I, SSS-III and SSS-XIV). Although these polysaccharides differ structurally and do not share antigenic determinants, the IR to all three is severely depressed. Antibody levels measured by radioimmunoassay to SSS-I and SSS-XIV were only 1/4 and to SSS-III 1/3 of the normal response. Antibody titres to DNP were 1/12 of those of normal mice. The number of direct PFC/spleen to DNP were reduced to 1/9 (817 vs 7747) and the indirect PFC to 1/16 (669 vs 10766) of the controls.
In contrast, mice bearing S37 (a pleomorphic neoplasm) had antibody levels against SSS-I and SSS-III which were equal to or higher than the controls and only somewhat reduced to SSS-XIV. Anti-DNP antibody titres were reduced to half in S37 bearing mice compared to controls (299 vs 691) while the number of indirect PFC/spleen were comparable. The number of direct anti-DNP PFC/spleen was even higher in the S37 bearing mice than in the controls (4849 vs 2646). However, direct and indirect PFC/10
6
nucleated spleen cells were reduced in both TEPC-183 and S37 bearing mice, reflecting both splenomegaly and a reduction in the number of antibody secreting cells/10
6
spleen cells.
Thus, TEPC-183 severely suppressed the IR to both the DNP-ligand and three pneumococcal polysaccharides, while the IR of mice bearing S37 was generally comparable or higher than the controls to both DNP and Pn-SSS. Possible mechanisms for the myeloma induced suppression are discussed. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Supported in part by Grant #1-RO-1-CA 14300-01A3 and NO AI 42541, and Grant-in-Aid from Temple University. |
ISSN: | 0019-2805 1365-2567 |