(E)-5-(2-bromovinyl)-2'-deoxyuridine-5'-triphosphate as a DNA polymerase substrate

• Published in: Nucleic acids research Vol. 9; no. 24; pp. 6985 - 6994
• Main Authors: Sági, J, Szabolcs, A, Szemzö, A, Otvös, L
• Format: Journal Article
• Language: English
• Published: England 21.12.1981
• Subjects: (E)-5-(2-bromovinyl)-2'-deoxyuridine-5'-triphosphate; Antiviral Agents - pharmacology; Deoxyribonucleotides - metabolism; Deoxyuracil Nucleotides - pharmacology; DNA - biosynthesis; DNA polymerase; DNA Polymerase I - antagonists & inhibitors; DNA Polymerase I - metabolism; DNA Replication - drug effects; DNA-Directed DNA Polymerase - metabolism; Escherichia coli; Herpesviridae - drug effects; Substrate Specificity; substrates; Thymine Nucleotides - metabolism; Virus Replication - drug effects
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/9.24.6985

Time course of incorporation and the effect of 5'-triphosphate of the selective antiherpetic agent (E)-5-(2-bromovinyl)-2'-deoxyuridine (bv5dUTP) on the incorporation of dTTP and dATP into template-primers of different structure were studied in E. coli DNA polymerase I Klenow fragment enzyme-catalyzed reactions. bv5dUTP could substitute for dTTP depending on the structure of template-primer. E.g. into calf thymus DNA incorporation of bv5dUMP was around 80% of that of dTMP at 30 minutes of incubation. The analog has also inhibited dTMP incorporation, net DNA synthesis, however, was hardly affected. The substrate properties of the analog were studied with [2-14C]-labelled bv5dUTP.