TRAF protein function in noncanonical NF-κB signaling

Nuclear factor-κB (NF-κB) signaling is classified into the canonical and noncanonical pathways. We describe in this chapter the methods used to study the noncanonical pathway, including derivation of primary cells, pathway stimulation, and immunoblotting.

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1280; p. 247
Main Authors Reichardt, Anna D, Pindado, Jose, Zaver, Shivam A, Cheng, Genhong
Format Journal Article
LanguageEnglish
Published United States 2015
Subjects
Animals
B-Lymphocytes - metabolism
Blotting, Western
Cell Culture Techniques
Enzyme Activation
Fibroblasts - metabolism
Immunomagnetic Separation - methods
Macrophages - metabolism
Mice
NF-kappa B - chemistry
NF-kappa B - metabolism
Primary Cell Culture
Protein Binding
Protein Transport
Signal Transduction
Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - metabolism
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Summary:Nuclear factor-κB (NF-κB) signaling is classified into the canonical and noncanonical pathways. We describe in this chapter the methods used to study the noncanonical pathway, including derivation of primary cells, pathway stimulation, and immunoblotting.
ISSN:1940-6029
DOI:10.1007/978-1-4939-2422-6_14