Antioxidant Effect of Taurine-Rich Paroctopus dofleini Extracts Through Inhibiting ROS Production Against LPS-Induced Oxidative Stress In Vitro and In Vivo Model

Taurine is an essential amino acid to improve the function of cardiovascular, skeletal muscle, retina, and central nervous system. It also plays a role as an antioxidant agent against reactive oxygen species (ROS) generated by various substances. The aim of the current study was to examine the antio...

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Bibliographic Details
Published inAdvances in experimental medicine and biology Vol. 975 Pt 2; p. 1165
Main Authors Kim, Yon-Suk, Kim, Eun-Kyung, Jeon, Nam-Joo, Ryu, Bo-Im, Hwang, Jin-Woo, Choi, Eun-Ju, Moon, Sang-Ho, Jeon, Byong-Tae, Park, Pyo-Jam
Format Journal Article
LanguageEnglish
Published United States 2017
Subjects
Animals
Antioxidants - pharmacology
Complex Mixtures - chemistry
Complex Mixtures - pharmacology
Embryo, Nonmammalian
Lipid Peroxidation - drug effects
Lipopolysaccharides - toxicity
Mice
Octopodiformes - chemistry
Oxidative Stress - drug effects
RAW 264.7 Cells
Reactive Oxygen Species - metabolism
Taurine - pharmacology
Zebrafish
Oxidative stress
Antioxidant effect
Paroctopus dofleini
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Summary:Taurine is an essential amino acid to improve the function of cardiovascular, skeletal muscle, retina, and central nervous system. It also plays a role as an antioxidant agent against reactive oxygen species (ROS) generated by various substances. The aim of the current study was to examine the antioxidant capacity of water extracts of Paroctopus dofleini. Radical scavenging activity of P. dofleini extracts was performed using an ESR spectrophotometer. Protective effects of P. dofleini extracts against lipopolysaccharide (LPS)-induced oxidative stress in RAW264.7 cells were evaluated using flow cytometry. The P. dofleini extracts showed a potent antioxidant activity against LPS-induced oxidative stress on RAW264.7 cells. Furthermore, the in vivo antioxidant activity of P. dofleini extract on LPS-induced oxidative stress was assessed using zebrafish embryos. P. dofleini successfully scavenged the LPS-induced intracellular ROS and prevented lipid peroxidation in zebrafish embryos. The results obtained in this study clearly demonstrate that the P. dofleini significantly scavenge the ROS and prevent lipid peroxidation in both in vitro and in vivo models.
ISSN:0065-2598
DOI:10.1007/978-94-024-1079-2_93