Isolation and properties of a new site-specific endonuclease Bme142I from Bacillus megaterium 142

A new type II restriction endonuclease, Bme142I, was partially purified and characterized from Bacillus megaterium 142. It has been seen in a crude extract, because this bacterial strain does not have significant amounts of contaminating nucleases. In contrast to its isoschisomer, the restriction en...

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Published inNucleic acids research Vol. 16; no. 21; p. 10399
Main Authors Fomenkov, A I, Kramarov, V M, Andreev, L V, Mochalov, V V, Smolyaninov, V V, Matvienko, N I
Format Journal Article
LanguageEnglish
Published England 11.11.1988
Subjects
Bacillus megaterium
Bacillus megaterium - enzymology
Base Sequence
Deoxyribonucleases, Type II Site-Specific - isolation & purification
Deoxyribonucleases, Type II Site-Specific - metabolism
Substrate Specificity
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Summary:A new type II restriction endonuclease, Bme142I, was partially purified and characterized from Bacillus megaterium 142. It has been seen in a crude extract, because this bacterial strain does not have significant amounts of contaminating nucleases. In contrast to its isoschisomer, the restriction endonuclease HaeII, which cleaves the same recognition site to produce a 4 base 3' extension, the Bme142I produces blunt-ended DNA fragments.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.21.10399