Screening for epigenetic target genes that enhance reprogramming using lentiviral-delivered shRNA

Small molecules will need to be identified and/or developed that target protein classes limiting reprogramming efficiency. A specific class of proteins includes epigenetic regulators that silence, or minimize expression, of pluripotency genes in differentiated cells. To better understand the role of...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 702; p. 299
Main Authors Rim, Jong S, Strickler, Karen, Barnes, Christian, Harkins, Lettie, Staszkiewicz, Jaroslaw, Power, Rachel, Eilertsen, Kenneth J
Format Journal Article
LanguageEnglish
Published United States 2011
Subjects
Adipocytes - cytology
Adipocytes - metabolism
Cells, Cultured
Cellular Reprogramming - genetics
Colony-Forming Units Assay
Dermis - cytology
DNA, Complementary - biosynthesis
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Epigenesis, Genetic
Fibroblasts - cytology
Fibroblasts - metabolism
Gene Transfer Techniques
Genes, Reporter
Genetic Testing - methods
Humans
Immunohistochemistry
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Lentivirus - genetics
Lung - cytology
Muscle Cells - cytology
Muscle Cells - metabolism
Muscle, Skeletal - cytology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Small Interfering - metabolism
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Summary:Small molecules will need to be identified and/or developed that target protein classes limiting reprogramming efficiency. A specific class of proteins includes epigenetic regulators that silence, or minimize expression, of pluripotency genes in differentiated cells. To better understand the role of specific epigenetic modulators in reprogramming, we have used shRNA delivered by lentivirus to assess the significance of individual epi-proteins in reprogramming pluripotent gene expression.
ISSN:1940-6029
DOI:10.1007/978-1-61737-960-4_22