DNA extraction from keratin and chitin

DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica-colum...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 840; p. 43
Main Authors Campos, Paula F, Gilbert, Thomas M P
Format Journal Article
LanguageEnglish
Published United States 01.01.2012
Subjects
Animals
Chitin - chemistry
Dithioerythritol - chemistry
DNA - isolation & purification
DNA - metabolism
Endopeptidase K - metabolism
Feathers - chemistry
Fossils
Hair - chemistry
Keratins - chemistry
Silicon Dioxide - chemistry
Online AccessGet more information

Cover

More Information
Summary:DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica-column or organic methods.
ISSN:1940-6029
DOI:10.1007/978-1-61779-516-9_6