Analysis of microtubule assembly kinetics using turbidimetry

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 777; p. 99
• Main Author: Gaskin, Felicia
• Format: Journal Article
• Language: English
• Published: United States 01.01.2011
• Subjects: Kinetics; Microtubules - metabolism; Nephelometry and Turbidimetry - methods; Tubulin - metabolism
• ISSN: 1940-6029
• DOI: 10.1007/978-1-61779-252-6_7

Turbidity measurements are rapid and reliable methods to follow the effects of drugs, proteins, nucleotides, metals, and other factors on the assembly kinetics of tubulin into microtubules in vitro and have been in use since 1974. Improvements in spectrophotometers and software have resulted in the...