Analysis of microtubule assembly kinetics using turbidimetry

Turbidity measurements are rapid and reliable methods to follow the effects of drugs, proteins, nucleotides, metals, and other factors on the assembly kinetics of tubulin into microtubules in vitro and have been in use since 1974. Improvements in spectrophotometers and software have resulted in the...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 777; p. 99
Main Author Gaskin, Felicia
Format Journal Article
LanguageEnglish
Published United States 01.01.2011
Subjects
Kinetics
Microtubules - metabolism
Nephelometry and Turbidimetry - methods
Tubulin - metabolism
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Summary:Turbidity measurements are rapid and reliable methods to follow the effects of drugs, proteins, nucleotides, metals, and other factors on the assembly kinetics of tubulin into microtubules in vitro and have been in use since 1974. Improvements in spectrophotometers and software have resulted in the use of less protein, and more curves can be analyzed continuously and almost simultaneously.
ISSN:1940-6029
DOI:10.1007/978-1-61779-252-6_7